Longitudinal bone, muscle and adipose tissue changes in physically active subjects – sex differences during adolescence and maturity

OBJECTIVES To explore changes in bone, muscle and adipose tissue composition in athletes with high physical activity levels at different stages of life. METHODS Thigh MRIs were acquired at baseline and 2-year follow-up for 20 young (16±1 years) and 20 mature (46±5 years) athletes (10 males, 10 females, respectively). Longitudinal changes in cross-sectional areas (CSAs) of femoral bone, quadriceps muscle, and thigh subcutaneous (SCF) and intermuscular (IMF) adipose tissue were evaluated. RESULTS Adolescent males displayed significant muscle (+5.0%, 95%CI: 0.8, 9.2) and bone growth (+2.9%, 95%CI: 1.3, 4.5), whereas adolescent females did not (muscle: +0.8%, 95%CI: -2.2, 3.8; bone: +1.9%, 95%CI: -2.1, 5.6). Adolescent and mature females showed significant SCF increases (+11.0%, 95%CI: 0.9, 21.1 and +6.0%, 95%CI: 0.6, 11.4, respectively), whereas adolescent and mature males did not (+7.2%, 95%CI: -8.0, 22.5 and +1.5%, 95%CI: -9.7, 11.8, respectively). Muscle and bone changes were highly correlated in adolescent males (r=0.66), mature males (r=0.75) and mature females (r=0.68) but not in adolescent females (r=-0.11). CONCLUSIONS The results suggest sex-specific patterns of age-related change in bone, muscle and adipose tissue, and tight coupling of bone and muscle growth. Sex-specific bone-muscle-adipose tissue relationships may have implications for understanding sex differences in fracture risk.